Vijay Reddy group (The Scripps Research Institute) and collaborators
The groups of Vijay Reddy and Glen Nemerow at the Scripps
Research Institute determined structures of two fiber knob proteins of
species B adenovirus (Pache, L., et al., Virology 375, 573-579 (2008); Pache,
L. et al., J.Virol. 82, 7923-7931 (2008)). Species B adenovirus is of
interest because it causes infections of the upper respiratory tract, as well
as of the kidney and urinary tract. But it is also under major investigation
for use as a gene delivery vector in medicine. A majority of species B
adenovirus (Ads) use the cellular receptor, CD46, as their primary receptor.
However, different fiber knobs of different Ads have different affinities for
CD46 binding, and the precise mechanisms involved in binding of different Ad
fiber knob types to CD46 are not well understood. The authors have
determined structures of Ad35 fiber knob, and of Ad16 fiber knob, and
compared these to a structure of a complex between Ad11 fiber knob and CD46.
All X-ray data were collected at GM/CA-CAT. These structures, along with
biochemical data, provide better understanding towards development of more
efficient Ad gene delivery vectors.
Figure: A ribbon diagram showing two
subunits of the Ad16 fiber knot (red) superimposed on the complex of the Ad11
fiber knob (green) and SCR1-SCR2 of CD46 (blue).
Pache, L, Venkataraman, S, Nemerow, GR, Reddy, VS. Conservation of fiber
structure and CD46 usage by subgroup B2 adenoviruses, Virology 375 (2),
573-579 (2008). DOI: 10.1016/j.virol.2008.02.013.|
Pache, L, Venkataraman, S, Reddy, VS, Nemerow, GR. Structural Variations in
Species B Adenovirus Fibers Impact CD46 Association, J. Virol. 82 (16),
7923-7931 (2008). DOI: 10.1128/JVI.00754-08.|