An improved, high capacity (18 puck, 288 pins) automounter, based on the
Berkeley Automounter (BAM), is implemented on both GM/CA ID beamlines.
|The high capacity automounter installed in the GM/CA 23-IDD hutch|
|View into the GM/CA High Capacity, 18-puck Dewar Letters indicate the corresponding
position in the template. The A and G positions are loaded with ALS pucks. A Unipuck
is loaded in the H-position. (Positions L through O are not in the picture)|
Automounter Equipment Requirements
Puck Set / Tools
Automounter Spreadsheets download
Automounter Spreadsheets filling instructions
The automounter requires a complete puck tool set and specially
sized bases and pins. Crystals will need to be pre-mounted in the
pucks prior to arrival for beamtime.
Puck Set / Tools
Pucks Sets can be ordered from:
Crystal Positioning Systems,
Qty. Item Price/Each
1 10" bent cryo tong
1 10" straight cryo tong
1 Puck pusher
1 Puck wand
7 Cryo puck
7 Magnetic cryo puck lid
1 Cryo puck shipping cane*
1 Puck separator tool
Total ~ approximately $3100
Loaner kits are available upon request.
*Shipping cane for Taylor Wharton CP100 shipping dewar or 34HC storage dewar.
We advise users to use ONLY "ALS style" or SPINE magnetic bases: either
Hampton CrystalCap Magnetic ALS (part number HR4-779-ALS) or
Hampton SPINE (part numbers HR8-xxx).
|Hampton CrystalCap Magnetic: ALS-style on the right
The above bases can be obtained at these sources:
The only pins which can be used with the robot are the "Hampton 18
mm" pins. This corresponds to breaking off the two end segments of a
The image below details the assembled ALS-style base+pin ("18 mm")
which is the preferred design for the automounter. The dimensions are fairly
critical here, and the most important one from the automounter's standpoint
is the distance from the inside mounting surface (which corresponds
with the top of the goniometer magnetic mount) to the center of the
crystal = 20.3mm ± 1mm.
It appears that the SSRL-style pins with the copper stem do not work well.
Automounter spreadsheets download
JBluIce requires a Microsoft Excel spreadsheet file with samples descriptions
to be loaded prior to using the automounter. The automounter templates are avalible
for downloading through this link or
after logging onto GM/CA computers, site files can be copied from the /px/data/
directory. The file names are:
Automounter spreadsheets filling instructions
The number of rows is used to determine which style puck is
loaded; so do not alter the number of rows. The spreadsheet may be populated
with information or left blank.
Only three of the columns present in the spreadsheet are used by the
screening system as described below. The rest of the columns are for
your reference only and will not be displayed in the JBluIce spreadsheet:
||refers to a port in the puck. The robot will use this port
to retrieve the sample. Don't change this column.
||this label is used by the screening system to generate filenames. It is
recommended that a unique ID be used for each sample.
||If provided, this field will be used to generate subdirectories for each
sample under the root screening directory. The default template Excel
spreadsheet uses the Port as the subdirectory name.
||Please do not put any spaces into the spreadsheet
filename or in Port, CrystalID and Directory columns. Instead, use
underscores or dashes when a separator is needed.
Additional optional columns can be used to provide prior information for
data processing and structure determination pipelines.
||defines a metal in the crystal, if defined, the data will be
processed as anomalous data.
||defines the PDB file to be used for ligand screening using DIMPLE.
The files must be located in Downloads or Desktop directory if only the
filename is given. Otherwise, the filename should be given in the full
||space group name (e.g. p21, p43212), defines a space group to be
used in data processing.
||define the sequence file to be used by MR, similarly defined as