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| New data - 1.4 Å (Diffraction was still strong at the edge of the detector) |
PDB 7L52 - 1.8 Å (2022 data, Advanced Photon Source, SBC, Sector 19) |
Left: New fixed-target serial crystallography data from GM/CA (23-ID-B). Crystal size 5 - 10 microns. Beam size 5 x 5 microns (FWHM) beam, ~2x10^13 photons/sec, 12 keV, 100 Hz frame rate, 100,000 frames, sample translation 0.5 mm/sec. Diffraction was still strong at the edge of the detector.
Right: Published structure from SBC (sector 19) before the upgrade: https://doi.org/10.1107/S1600577522007895. Beam size 50 x 50 micron (FWHM), 3x10^12 photons/sec, 12.66 keV, 20 Hz frame rate, 38,500 frames. The paper and reference for crystallization do not report the crystal size.
The red ellipse is circling a disulfide bond. The bond is broken on the right due to radiation damage, while it remains intact on the left.
Acknowledgements:
SBC and eBERLight:
Natalia Maltseva, Priyanka Gade, Darren Sherrell, Alex Lavens, Karolina
Michalska, and Andrzej Joachimiak for providing the samples.
On March 11, 2025, we began scientific commissioning. During the APS shutdown to replace the storage ring, we also rebuilt the GM/CA beamlines. The X-ray beam is very small and extremely bright!
On November 25, 2024, we received permission to begin technical commissioning of the new optics and endstation instruments with X-rays for GM/CA@APS beamlines. Technical commissioning of beamline 23-ID-B began immediately. An intense, focused sub-micron beam was achieved, and before the APS shutdown (December 19 - January 26), we began testing as many components as possible. Just before the shutdown, we performed "heroic" experiments under manual conditions and obtained high-quality electron-density maps (see below). The current focus is on transitioning from these proof-of-principle experiments to user-friendly beamline operability as soon as possible. This is a complex process because we replaced many outdated or end-of-life components on the beamline, including the focusing optics, the beam-delivery system, the goniometry, and the low-level hardware and software that drives all motions on the beamline and the data acquisition system. See this website for future announcements.
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Figure:
Left: trypsin (1.0-Å resolution, 50µm beam, 20 keV X-rays).
Right: crambin (0.7-Å resolution, 1µm beam, 20 keV X-rays).
2Fo-Fc density (blue) is contoured at 1.0 σ for trypsin and 1.5 σ
for crambin, and Fo-Fc density at 3 σ for both in green (+) and red (-).
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GM/CA @ APS
