Synchrotron Serial Crystallography Available at the GM/CA @ APS
Synchrotron serial crystallography experiments are now available to interested users at GM/CA@APS.
Samples can be delivered to the x-ray beam via either:
- A Lipidic Cubic Phase (LCP) injector
- A variety of fixed target systems
GM/CA@APS staff have collaborated with user groups to prove the
feasibility at beamlines 23IDB and 23IDD. Structures have been
determined with an LCP injector from Arizona State University (ASU), as
well as with several different types of fixed targets at room temperature
and at cryogenic temperature. Background information and publications
are listed below. For those interesting in using the LCP injector,
please contact Dr. David Kissick ([email protected]); for those
interested in employing fixed targets, please contact
Dr. Craig M. Ogata.
|ASU Injector (in house) at the GM/CA 23-ID-D beamline:
(a) Schematic diagram of the setup.
(b) LCP injector (Weierstall et al., 2014) mounted on translation
stages (not shown). The catcher is also shown.
(c) View of the LCP stream extruding out of a 50 mm glass capillary nozzle.
The intersection point of the two white dashed lines indicates the position
of the X-ray beam. Reference:
J.M. Martin-Garcia, C.E. Conrad, G. Nelson, N. Stander, N.A. Zatsepin,
J. Zook, L. Zhu, J. Geiger, E. Chun, D. Kissick, M. Hilgart, C. Ogata,
A. Ishchenko, N. Nagaratnam, S. Roy-Chowdhury, J. Coe, G. Subramanian,
A. Schaffer, D. James, G. Ketwala, N. Venugopalan, S. Xu, S. Corcoran,
D. Ferguson, U. Weierstall, U., J.C.H. Spence, V. Cherezov, P. Fromme,
R.F. Fischetti, W. Liu, IUCrJ, 4, 439-454 (2017).
Rapid automated analysis of thousands of sparse images.
|Long tapered beamstop:
10x background reduction.
|Highly sensitive raster scans of a fixed targets via JBluIce GUI.
Reference: J. Broecker, T. Morizumi, W.-L. Ou, V. Klingel, A. Kuo,
D.J. Kissick, A. Ishchenko, M.-Y. Lee, S. Xu, O. Makarov, V. Cherezov,
C.M. Ogata, O.P. Ernst, Nature Protocols 13(2), 260-292 (2018)..